生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
hamster, human, mouse
技術
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... RLIM(51132)
一般說明
E3 ubiquitin-protein ligase RLIM (UniProt Q9WTV7; also known as Hoxa1 regulated, LIM domain-interacting RING finger protein, R-LIM, RING finger LIM domain-binding protein, Ring finger protein 12) is encoded by the Rlim (also known as AL022832, AW743871, Ha1r, Rlim1, Rnf12) gene (Gene ID 19820) in murine species. The CLIM/LDB/NLI and RLIM/RNF12 cofactors are transcriptional coregulatory proteins characterized by their ability to bind the LIM domains of nuclear LIM proteins, including LIM homeodomain (LIM-HD) transcription factors. The association of CLIM with LIM-HDs positively regulate their biological and transcriptional activity. On the other hand, the RING finger ubiquitin ligase RLIM acts as a negative regulator of LIM-HD activity by interacting with and targeting CLIM for degradation via the ubiquitin-proteasome pathway.
特異性
Exhibits no cross-reactivity toward the homologous ubiquitin ligase Rnf6.
免疫原
Recombinant protein corresponding to mouse RLIM/Rnf12.
應用
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-RLIM/Rnf12 Antibody is validated for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunofluorescence for the detection of RLIM/Rnf12.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot selectively detected endogenouse RLIM in HEK293 lysate and in vitro transcribed and translated (TNT) RLIM, but not TNT Rnf6.(Courtesy of Dr. Ingolf Bach, University of Massachusetts Medical School).
Western Blotting Analysis: A representative lots detected RLIM in cell and tissue lysates of human, murine, and Chinese hamster species (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096; Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981; Gungor, C., et al. (2007). Proc. Natl. Acad. Sci. USA. 104(38):15000-15005; Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791; Kramer, O.H., et al. (2003). EMBO. 22(13):3411-3420; Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunocytochemistry Analysis: A representative lots detected RLIM in cultured murine mammary epithelial cells and MEFs by fluorescent immunocytochemistry (Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunocytochemistry Analysis: A representative lot detected predominant nuclear expression of RLIM in HeLa, MCF-7, HEK293, and murine pituitary gonadotroph Alpha T3 cells by fluorescent immunocytochemistry (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Chromatin Immunoprecipitation Analysis: A representative lots detected RLIM association at the alphaGSU promoter in murine pituitary gonadotroph Alpha T3 cells, as well as estrogen-induced RLIM recruitment to the estrogen response element (ERE) in the promoter region of PS2 and WISP2 in MCF-7 cells (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103; Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunohistochemistry Analysis: A representative lot detected similar stage-dependent expression patterns between RLIM and CLIM1/2 in the developing neural tube during early mouse embryogenesis (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).
Immunohistochemistry Analysis: A representative lot detected a RLIM expression pattern distinct from that of the homologous ubiquitin ligase Rnf6 in E12.5-E13 murine embryonic spinal cord (Tursun, B., et al. (2005). Genes Dev. 19(19):2307-2319).
Immunohistochemistry Analysis: A representative lot detected RLIM-positive cells in breast epidermis and oral cavity epithelium using paraffin-embedded adult human tissue sections (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Immunohistochemistry Analysis: A representative lot detected RLIM accumulation during oocyte maturation using paraffin-embedded ovary sections from female mice (Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with ERalpha in frozen human breast tumor sections by dual fluorescent immunohistochemistry (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated CLIM and ERalpha with RLIM from MCF-7 lysates (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with H3K27me3 immunoreactivity in paraffin-embedded E7-7.5 mouse embryos by dual fluorescent immunohistochemistry (Shin, J., et al. (2014). Nature. 511(7507):86-89).
Western Blotting Analysis: A representative lots detected RLIM in cell and tissue lysates of human, murine, and Chinese hamster species (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096; Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981; Gungor, C., et al. (2007). Proc. Natl. Acad. Sci. USA. 104(38):15000-15005; Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791; Kramer, O.H., et al. (2003). EMBO. 22(13):3411-3420; Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunocytochemistry Analysis: A representative lots detected RLIM in cultured murine mammary epithelial cells and MEFs by fluorescent immunocytochemistry (Jiao, B., et al. (2012). Cell. 149(3):630-641; Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunocytochemistry Analysis: A representative lot detected predominant nuclear expression of RLIM in HeLa, MCF-7, HEK293, and murine pituitary gonadotroph Alpha T3 cells by fluorescent immunocytochemistry (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Chromatin Immunoprecipitation Analysis: A representative lots detected RLIM association at the alphaGSU promoter in murine pituitary gonadotroph Alpha T3 cells, as well as estrogen-induced RLIM recruitment to the estrogen response element (ERE) in the promoter region of PS2 and WISP2 in MCF-7 cells (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103; Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunohistochemistry Analysis: A representative lot detected similar stage-dependent expression patterns between RLIM and CLIM1/2 in the developing neural tube during early mouse embryogenesis (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).
Immunohistochemistry Analysis: A representative lot detected a RLIM expression pattern distinct from that of the homologous ubiquitin ligase Rnf6 in E12.5-E13 murine embryonic spinal cord (Tursun, B., et al. (2005). Genes Dev. 19(19):2307-2319).
Immunohistochemistry Analysis: A representative lot detected RLIM-positive cells in breast epidermis and oral cavity epithelium using paraffin-embedded adult human tissue sections (Jiao, B., et al. (2013). Mol Biol Cell. 24(19):3085-3096).
Immunohistochemistry Analysis: A representative lot detected RLIM accumulation during oocyte maturation using paraffin-embedded ovary sections from female mice (Shin, J., et al. (2010). Nature. 467(7318):977-981).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with ERalpha in frozen human breast tumor sections by dual fluorescent immunohistochemistry (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated CLIM and ERalpha with RLIM from MCF-7 lysates (Johnsen, S.A., et al. (2009). Cancer Res. 69(1):128-136).
Immunofluorescence Analysis: A representative lot detected RLIM nuclear co-localization with H3K27me3 immunoreactivity in paraffin-embedded E7-7.5 mouse embryos by dual fluorescent immunohistochemistry (Shin, J., et al. (2014). Nature. 511(7507):86-89).
品質
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:5,000 of this antibody detected RLIM/Rnf12 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: A 1:5,000 of this antibody detected RLIM/Rnf12 in 10 µg of mouse brain tissue lysate.
標靶描述
~69 kDa observed. Uncharacterized band(s) may appear in some lysates.
外觀
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Elsa J Sousa et al.
Cell stem cell, 22(6), 919-928 (2018-05-29)
A hallmark of naive pluripotency is the presence of two active X chromosomes in females. It is not clear whether prevention of X chromosome inactivation (XCI) is mediated by gene networks that preserve the naive state. Here, we show that
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