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Merck
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文件

AB3202

Sigma-Aldrich

抗-LIM-3 抗体

Chemicon®, from rabbit

别名:

Anti-HMFN1661

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

fish, frog, mouse, human

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... ABLIM3(22885)

特異性

可识别LIM-3。该抗体不与组织切片中的gsh-4交叉反应,但是在蛋白质印迹和免疫沉淀中可能有交叉反应。

免疫原

小鼠LIM-3蛋白的C末端部分。

應用

免疫组化:在石蜡包埋的切片上为1:250-1:1,000 。在冰冻切片上为1:3,000-1:6,000 。在全标本上为1:4,000-1:8,000。推荐固定剂MEMFA.

免疫细胞化学:1:200-1:500

蛋白质印迹:1:2,500-1:5,000

免疫沉淀:1:200

该抗体在免疫荧光中工作正常。使用偶联生物素的二抗,然后以链霉亲和素偶联至荧光染料。

最佳工作稀释度必须由最终用户进行确定。
抗LIM-3抗体是针对LIM-3的抗体,可用于IP、WB、IC、IH、IH(P)。
研究子类别
发育神经科学

神经&胶质标志物
研究类别
神经科学

外觀

形式:纯化
纯化的免疫球蛋白。 0.02M磷酸盐缓冲液,0.25M NaCl和0.1%叠氮化钠的液体。

儲存和穩定性

以未稀释的等分试样保存于2-8°C下6个月。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Mai Nakamura et al.
Cytotechnology, 68(3), 409-417 (2014-10-31)
Mouse embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have the ability to differentiate in vitro into various cell lineages including neurons. The differentiation of these cells into neurons has potential applications in regenerative medicine. Previously, we reported
Clifford R Hume et al.
Gene expression patterns : GEP, 7(7), 798-807 (2007-07-03)
A cascade of transcription factors is believed to regulate the coordinate differentiation of primordial inner ear cells into the subtypes of hair cells and supporting cells. While candidate genes involved in this process have been identified, the temporal and spatial
Stephanie W Fowler et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(23), 7871-7885 (2014-06-06)
An unresolved debate in Alzheimer's disease (AD) is whether amyloid plaques are pathogenic, causing overt physical disruption of neural circuits, or protective, sequestering soluble forms of amyloid-β (Aβ) that initiate synaptic damage and cognitive decline. Few animal models of AD
Zhiyong Liu et al.
PloS one, 9(2), e89377-e89377 (2014-03-04)
Regeneration of auditory hair cells (HCs) is a promising approach to restore hearing. Recent studies have demonstrated that induced pluripotent stem cells/embryonic stem cells or supporting cells (SCs) adjacent to HCs can be converted to adopt the HC fate. However
Anne-Laure Schang et al.
Molecular endocrinology (Baltimore, Md.), 27(1), 74-91 (2012-12-06)
GATA2 transcription factor and LIM homeodomain proteins Islet1 (ISL1) and LIM homeobox 3 (LHX3) are suspected to be involved in gonadotrope cell fate and maintenance. The GnRH receptor gene (Gnrhr), crucial for gonadotrope function, is expressed in the pituitary gland

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