推荐产品
生物源
human
品質等級
重組細胞
expressed in baculovirus infected Sf21 cells
形狀
liquid
比活性
377 U/mg
製造商／商標名
Upstate®
技術
activity assay: suitable (kinase)
溶解度
water: soluble
NCBI登錄號
UniProt登錄號
儲存溫度
−70°C
基因資訊
human ... MTOR(2475)
一般說明
Research area: Cell Signaling
Protein Target: mTOR (FRAP1)
Target Sub-Family: Lipid/Atypical
N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end
The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.
Protein Target: mTOR (FRAP1)
Target Sub-Family: Lipid/Atypical
N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end
The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.
應用
The active mTOR (1362-end) protein, 10 µg, has been used in the mTOR substrate phosphorylation assay to validate the biochemical and pharmacological profile of a novel mTOR-KI for targeting mTOR signaling pathways in vitro and in vivo.
生化／生理作用
Mechanistic target of rapamycin (mTOR) plays a pivotal role in regulating various essential cellular processes, ranging from protein synthesis to autophagy. Moreover, it influences metabolism, cellular survival, gene transcription, and cytoskeletal components. It gets activated in response to growth factors, nutrients, mitogens, and hormones.Downregulation of mTOR signaling is associated with the advancement of cancer and diabetes, and aging process.
包裝
也有100 μg规格，请查询价格和供货情况。
單位定義
比活：其中一个单位的mTOR活性定义为在30°C下每分钟将1 nmole磷酸盐掺入2 mg/ml mTOR底物中，最终ATP浓度为100 µM。
外觀
在100 µL 10mM HEPES、50mM NaCl、50 mMβ-甘油磷酸盐、0.25 mM原钒酸钠、10 mM NaF、300 µg/mL FLAGTM肽、20%v/v甘油中加入16 µg酶
儲存和穩定性
储存在-70°C。 为了最大程度地回收产品，在取下盖子之前，将原始样品瓶进行离心。
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
Jianchang Qian et al.
Oncotarget, 7(41), 67071-67086 (2016-08-27)
The mechanistic target of rapamycin (mTOR) is a rational target for cancer treatment. While the mTORC1-selective rapalogs have shown significant benefits in the clinic, antitumor response may be further improved by inhibiting both mTORC1 and mTORC2. Herein, we established target
mTOR: Exploring a New Potential Therapeutic Target for Stroke
Castellanos M, et al.
Molecules to Medicine with mTOR, 105-122 (2016)
mTOR Signaling in Growth, Metabolism, and Disease
Saxton RA and Sabatini DM
Cells, 168(6), 960?976- 960?976 (2017)
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