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生物源
mouse
品質等級
重組細胞
expressed in E. coli
分子量
Mw 67.8 kDa
製造商／商標名
Upstate®
技術
activity assay: suitable (kinase)
NCBI登錄號
UniProt登錄號
一般說明
N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
Product Source: recombinant, mouse, full-length MAP Kinase 2
生化／生理作用
Protein Target: MAPK2
Target Sub-Family: CMGC
包裝
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.
品質
Routinely evaluated by phosphorylation myelin basic protein
外觀
Glutathione agarose; Ni2+/NTA agarose
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
儲存和穩定性
Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.
其他說明
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.
Sanghera, J S, et al.
Biochimica et Biophysica Acta, 1095, 153-160 (1991)
X Yang et al.
The Journal of biological chemistry, 273(45), 29879-29887 (1998-10-29)
The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in virus replication and infectivity. Here we show that Vif is phosphorylated and regulated by p44/42 mitogen-activated protein kinase (MAPK). Vif phosphorylation by MAPK was demonstrated in
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
Sanghera, J S, et al.
The Journal of Biological Chemistry, 265, 52-57 (1990)
B Zhang et al.
The Journal of biological chemistry, 271(50), 31771-31774 (1996-12-13)
Peroxisome proliferator-activated receptor (PPAR) gamma plays an important role in adipocyte differentiation and the regulation of adipocyte gene expression. Insulin also serves to promote adipogenesis. We report that insulin and a PPARgamma ligand (thiazolidinedione (TZD)) stimulate in a synergistic manner
alpha7 Nicotinic Acetylcholine Receptors Mediate {beta}-Amyloid Peptide-induced Tau Protein Phosphorylation
Wang, H. Y., et al
The Journal of Biological Chemistry, 278, 31547-31553 (2003)
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