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  • Cellular uptake of covalent poly(allylamine hydrochloride) microcapsules and its influences on cell functions.

Cellular uptake of covalent poly(allylamine hydrochloride) microcapsules and its influences on cell functions.

Macromolecular bioscience (2012-09-26)
Bing Wang, Yuying Zhang, Zhengwei Mao, Changyou Gao
ABSTRACT

The mechanism of the cellular uptake of polyelectrolyte microcapsules and its influences on the functions and toxicity of human SMCs are explored. The covalently assembled poly(allylamine hydrochloride)/glutaraldehyde microcapsules are easily ingested by SMCs mainly through macropinosis and caveolae-mediated endocytosis pathways. The capsules mainly disperse in cytoplasm without colocalization in early endosomes and cell nucleus. The results of gene chips reveal substantial and profound alternation of cell phenotypes and functions. Uptake of the microcapsules cause a slight decrease of cell viability, but leads to significant changes in cytoskeleton organization, cell cycle, as well as cell adhesion and migration ability.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(allylamine hydrochloride), average Mw ~17,500 (GPC vs. PEG std.)
Pricing and availability is not currently available.
Sigma-Aldrich
Poly(allylamine hydrochloride), average Mw 50,000
Pricing and availability is not currently available.
Sigma-Aldrich
Poly(allylamine) solution, 20 wt. % in H2O
Pricing and availability is not currently available.
Sigma-Aldrich
Poly(allylamine) solution, average Mw ~65,000, 10 wt. % in H2O
Pricing and availability is not currently available.