- In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus.
In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus.
Molecular therapy : the journal of the American Society of Gene Therapy (2019-04-10)
Rianne N Esquivel, Ami Patel, Sagar B Kudchodkar, Daniel H Park, Karin Stettler, Martina Beltramello, Jeffrey W Allen, Janess Mendoza, Stephanie Ramos, Hyeree Choi, Piyush Borole, Kanika Asija, Mamadou Bah, Shareef Shaheen, Jing Chen, Jian Yan, Amy C Durham, Trevor R F Smith, Kate Broderick, Ghiabe Guibinga, Kar Muthumani, Davide Corti, Laurent Humeau, David B Weiner
PMID30962164
ABSTRACT
Zika virus (ZIKV) infection is endemic to several world regions, and many others are at high risk for seasonal outbreaks. Synthetic DNA-encoded monoclonal antibody (DMAb) is an approach that enables in vivo delivery of highly potent mAbs to control infections. We engineered DMAb-ZK190, encoding the mAb ZK190 neutralizing antibody, which targets the ZIKV E protein DIII domain. In vivo-delivered DMAb-ZK190 achieved expression levels persisting >10 weeks in mice and >3 weeks in non-human primate (NHPs), which is protective against ZIKV infectious challenge. This study is the first demonstration of infectious disease control in NHPs following in vivo delivery of a nucleic acid-encoded antibody, supporting the importance of this new platform.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Anti-Human IgG (H+L)-Peroxidase antibody produced in donkey, affinity isolated antibody, lyophilized powder