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G004710

HPLC Analysis of FMOC-Proline Enantiomers on Astec® CYCLOBOND I 2000 SN

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CYCLOBOND I 2000 SN, 25 cm x 4.6 mm I.D., 5 μm particles (20524AST)
mobile phase: [A] acetonitrile; [B] methanol; [C] acetic acid; [D] ammonium hydroxide; (85:15:0.4:0.1, A:B:C:D)
flow rate: 0.9 mL/min
column temp.: 25 °C
detector: UV, 254 nm
injection: 5 μL
sample: 2 mg/mL in methanol

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

standard

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