ZRB2347

Anti-PON2 Antibody, clone 1B6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): A-esterase 2, Aromatic esterase 2, EC:3.1.1.2, Serum aryldialkylphosphatase 2, Serum paraoxonase/arylesterase 2
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B6, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• purified by: using Protein A
• species reactivity: rat, human
• species reactivity (predicted by homology): rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_000296
• UniProt accession no.: Q15165-2
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... PON2(5445)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Serum paraoxonase/arylesterase 2 (PON2). It targets an epitope within 17 amino acids from the N-terminal half. Predicted to react with all three isoforms.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human serum paraoxonase/arylesterase 2 (PON2).

Application

Quality Control Testing

Evaluated by Western Blotting in U87-MG cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PON2 in U87-MG cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PON2 in HeLa cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PON2 in Human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound PON2 peptide with a KD of 3.5 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PON2 in U-87 MG cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Serum paraoxonase/arylesterase 2 (UniProt: Q15165; also known as EC:3.1.1.2, PON 2, Aromatic esterase 2, A-esterase 2, Serum aryldialkylphosphatase 2) is encoded by the PON2 gene (Gene ID: 5445) in human. The paraoxonase (PON) gene family in humans consists of three conserved members, PON1, PON2, and PON3. They exhibit high similarity in their structural characteristics and have about ∼65% identity in their amino acids. PON2 is a membrane and peripheral membrane associated enzyme that is widely expressed with highest levels in liver, lung, placenta, testis, and heart tissue. It is capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters and exhibits  antioxidant activity. It serves as an intracellular guardian against oxidative stress. It is shown to prevent LDL lipid peroxidation and reverse the oxidation of mildly oxidized LDL (MM-LDL). It inhibits the ability of MM-LDL to induce monocyte chemotaxis. PONS2 overexpressing cells exhibit significantly less intracellular oxidative stress following treatment with hydrogen peroxide or oxidized phospholipid. These cells are also less effective in oxidizing and modifying LDL. PON2 is also involved in modulation of ER stress and regulation of cell proliferation/apoptosis. During ER stress, PON2 reduces the induction of proapoptotic CHOP protein, particularly the protein kinase JNK pathway, and also protects intrinsic apoptotic signaling, by prevention of mitochondrial superoxide formation and caspase activation. Three isoforms of PON2 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals.  (Ref.: Martinelli, N., et al. (2013).  Adv. Clin. Chem. 59; 65-100;  Draganov, DI., et al. (2005). J. Lipid Res. 46(6); 1239-1247; Ng, CJ., et al. (2001). J. Biol. Chem. 276(48); 44444-44449).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable