P7609
Anti-Serine/Threonine Protein Phosphatase 1γ1 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~36 kDa
species reactivity
human, mouse, bovine, rat
technique(s)
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:500-1:1,000 using total rat brain homogenate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... PPP1CC(5501)
mouse ... Ppp1cc(19047)
rat ... Ppp1cc(24669)
General description
Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. PP1 isoforms catalyze a wide range of protein dephosphorylation reactions in a tightly regulated manner and is expressed abundantly in the brain. PP1 has broad functions covering glycogen metabolism, protein synthesis, cell cycle and growth and muscle contractility. PP1 forms exclusive complexes with >50 regulatory subunits that allows for restricted subcellular location and thereby distinct cellular functions. There are 3 isoforms of PP1 (α, β and γ1) with 90% homology. PP1γ1 is expressed in brain and has been reported to be crucial for cell proliferation in lung carcinoma cells, A549. .
The antibody specifically recognizes protein phosphatase 1γ1 isoform.
The antibody specifically recognizes protein phosphatase 1γ1 isoform.
Immunogen
synthetic peptide (TPPRGMITKQAKK) corresponding to the C-terminus of the protein phosphatase 1γ1 catalytic subunit (amino acid residues 311-323).
Application
Anti-Serine/Threonine Protein Phosphatase 1γ1 antibody may be used for detection by immunoblotting in total rat brain homogenate at a working dilution of 1:500-1:1000. It is suitable for immunoprecipitation and protein microarray applications.
Physical form
Solution in phosphate buffered saline with 0.08% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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A Cheng et al.
The Journal of biological chemistry, 275(3), 1846-1854 (2000-01-15)
In lower eukaryotic organisms, the loss of serine/threonine protein phosphatase type 1 (PP1) results in growth arrest after the onset of mitosis. In humans, four highly homologous isoforms of PP1 (PP1alpha, PP1delta, PP1gamma1, and PP1gamma2) have been identified. Determining the
S Strack et al.
The Journal of comparative neurology, 413(3), 373-384 (1999-09-29)
Protein phosphatase 1 (PP1) is a gene family with a number of important functions in brain. Association with a wide variety of regulatory/targeting subunits is thought to be instrumental in directing the phosphatase to specific subcellular locations and substrates. By
Patricia T W Cohen
Journal of cell science, 115(Pt 2), 241-256 (2002-02-13)
Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits. Most of these target
Mathieu Bollen et al.
Trends in biochemical sciences, 35(8), 450-458 (2010-04-20)
Protein Ser/Thr phosphatase-1 (PP1) catalyzes the majority of eukaryotic protein dephosphorylation reactions in a highly regulated and selective manner. Recent studies have identified an unusually diversified PP1 interactome with the properties of a regulatory toolkit. PP1-interacting proteins (PIPs) function as
Protein phosphatases: recent progress.
S Shenolikar et al.
Advances in second messenger and phosphoprotein research, 23, 1-121 (1991-01-01)
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