P5400
Pectinesterase from orange peel
lyophilized powder, ≥150 units/mg protein
Synonym(s):
Pectin methylesterase, Pectin pectylhydrolase
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About This Item
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form
lyophilized powder
specific activity
≥150 units/mg protein
composition
Protein, 20-50% biuret
greener alternative product characteristics
Design for Energy Efficiency
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sustainability
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greener alternative category
, Enabling
storage temp.
2-8°C
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.
Biochem/physiol Actions
Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
Unit Definition
One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.
Physical form
Contains (NH4)2SO4 and sodium chloride
Analysis Note
Protein determined by biuret.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kirk E Francis et al.
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Arabidopsis (Arabidopsis thaliana) QUARTET (QRT) genes are required for pollen separation during normal floral development. In qrt mutants, the four products of microsporogenesis remain fused and pollen grains are released as tetrads. In Arabidopsis, tetrad analysis in qrt mutants has
Pectinesterase Activity and Proximate Analyses of Sea Buckthorn Juices
Harrison JE and Beveridge THJ
Journal of Food Science, 71(1), C23-C27 (2006)
Shoko Hongo et al.
The Plant cell, 24(6), 2624-2634 (2012-06-14)
Secondary cell walls, which contain lignin, have traditionally been considered essential for the mechanical strength of the shoot of land plants, whereas pectin, which is a characteristic component of the primary wall, is not considered to be involved in the
Alexis Peaucelle et al.
Current biology : CB, 18(24), 1943-1948 (2008-12-23)
Plant organs are produced from meristems in a characteristic pattern. This pattern, referred to as phyllotaxis, is thought to be generated by local gradients of an information molecule, auxin. Some studies propose a key role for the mechanical properties of
On-line characterization using ultrasound of pectin hydrolysis catalyzed by the enzyme pectinmethylesterase.
Cristina A, et al.
IOP Conference Series: Materials Science and Engineering, 42 (2012)
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