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Roche

Biotin-16-ddUTP

≥98% (HPLC), solution, 1 mM, suitable for hybridization

Synonym(s):

Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate

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About This Item

Empirical Formula (Hill Notation):
C32H52N7O17P3S
CAS Number:
188755-42-2
Molecular Weight:
931.78
UNSPSC Code:
41116100

Quality Level

100

Assay

≥98% (HPLC)

form

solution

mol wt

955.5 (biotin-16-ddUTP-Li4)

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

concentration

1 mM

technique(s)

hybridization: suitable

storage temp.

−20°C

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General description

Biotin-16-ddUTP, tetralithium salt; 1mM

Application

  • Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
  • DNA polymerase I (Holoenzyme and Klenow fragment)
  • T4 DNA polymerase
  • Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.
  • The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
  • Colony and plaque screening
  • In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.

Features and Benefits

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

Quality

Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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