HPLC Analysis of Chlorthalidone Enantiomers on Astec^® CYCLOBOND I 2000 RSP

column

CYCLOBOND I 2000 RSP, 25 cm x 4.6 mm I.D., 5 μm particles (20324AST)

mobile phase

[A] 1% triethylamine acetate, pH 4.1; [B] acetonitrile; (95:5, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

2 mg/mL in methanol