HPLC Analysis of 3-Methylaspartic Acid Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] methanol; [B] water; [C] formic acid; (80:20:0.02, A:B:C)

flow rate

0.9 mL/min

column temp.

25 °C

detector

ELSD

injection

4 μL

sample

5 mg/mL in methanol