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Cloning & Expression

Investigating your gene of interest requires reliable reagents and resources for molecular cloning, transfection, and protein expression workflows. Our efficient restriction and DNA modifying enzymes and protein expression vector technologies are designed to ensure an effective cloning and protein expression strategy. In addition, our lipid and polymer-based transfection reagents are suitable for delivering DNA, siRNA, miRNA, and CRISPR/Cas components into the cell type best suited for your needs. Explore our expansive portfolio of cloning and protein expression reagents and select the tools you need to make your next breakthrough.   

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Six laboratory bottles arranged in two rows. The back row features a larger bottle with a blue cap labeled “X-tremeGENE 360 Transfection Reagent,” including product details and storage instructions. In front, there are five smaller bottles with white caps, each labeled with different codes: D4072, D4073, D4074, D4075, and D4076.

Transfection

Transfection reagents, protocols, and resources including Roche X-tremeGENE transfection reagents for DNA, siRNA, miRNA, and CRISPR/Cas9 gene editing components.

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Molecular Cloning & Protein Expression

Molecular cloning and protein expression methods and protocols for reliable cloning and robust protein expression from insect, bacteria, and mammalian protein expression vectors.

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A circular diagram representing a plasmid map with various labeled elements. At the top, there’s a segment labeled “CMV promoter” in dark blue, followed by an orange segment labeled “hGh polyA.” To the right, there’s a yellow segment labeled “SV40 origin.” Below that, on the right side of the circle, is a green segment labeled “amp^r,” indicating ampicillin resistance. On the bottom left is another antibiotic resistance label, “amp^k,” in red. There are two more segments: one labeled “f1 origin” in light blue on the left and another labeled “pBR322 origin” at the bottom center in purple. The title above reads ‘MCS Regions Shown Below,’ indicating that these are multiple cloning sites within the plasmid used for genetic engineering purposes.
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