SRP6522
Ubiquitin-TAMRA-labeled (TMR-Ub)
≥95% (SDS-PAGE)
Synonym(s):
CEP80, HUBCEP80, RPS27A, Ribosomal Protein S27a, UBA80, UBB, UBCEP1, UBCEP80
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Assay
≥95% (SDS-PAGE)
form
liquid
mol wt
9.133 kDa
packaging
pkg of 50 μg
concentration
8 mg/mL
shipped in
dry ice
storage temp.
−70°C
General description
Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, TheTAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.
Physical form
≥ 8 mg/mL in PBS.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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