Schenk and Hildebrandt Vitamin 100 × (S3766) and Schenk and Hildebrandt Basal Salt Mixture (S6765) are chemstocks that may be used to generate Schenk and Hildebrandt (SH) medium for use in plant cell culture and microproagation.
Quantity
Package prepares 1 L of a 100× solution.
Reconstitution
Use at a concentration of 10 mL per 1 L of prepared medium to achieve the proper final concentration.
Six-year-old Levisticum officinale (lovage) hairy root cultures were used to study the effect of eight different NH(4) (+):NO(3) (-) ratios on their growth and volatile components. All cultures were kept at 24 degrees C on orbital shakers at 80 rpm
Methods in molecular biology (Clifton, N.J.), 547, 263-273 (2009-06-13)
Hairy root clones of Rehmannia glutinosa were established via transformation with Agrobacterium rhizogenes ATCC15834. To optimize the culturing conditions for both root growth and catalpol production, the effects of various combinations of seven basal media, pH, and carbon sources were
Methods in molecular biology (Clifton, N.J.), 547, 107-115 (2009-06-13)
This chapter describes culture conditions for high-frequency plant regeneration via somatic embryogenesis and cryopreservation from cell suspension cultures of Ranunculus kazusensis. Zygotic embryos form white nodular structures and pale-yellow calli at a frequency of 84.9% on half-strength Schenk and Hildebrandt
The biotransformation capacity of Levisticum officinale W.D.J. Koch hairy root cultures was studied by evaluating the effect of the addition of 25 mg/L menthol or geraniol on morphology, growth, and volatiles production. L. officinale hairy root cultures were maintained for
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