This product is supplied as a neat or pure liquid with a density of 1.07 g/mL and an average molecular weight of 625 g/mol. Assuming 100%, the molarity is 1.712 M. To make 1 L of a 50 mM solution from this, use 29.2 mL of the 1.712 M starting concentration.
X100
Triton™ X-100
Triton™ X-100
laboratory grade
Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
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5 ML
391,00 kr
100 ML
674,00 kr
500 ML
875,00 kr
1 L
1 230,00 kr
1 GAL
3 600,00 kr
5 GAL
14 760,00 kr
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5 ML
391,00 kr
100 ML
674,00 kr
500 ML
875,00 kr
1 L
1 230,00 kr
1 GAL
3 600,00 kr
5 GAL
14 760,00 kr
About This Item
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
UNSPSC Code:
12161900
PubChem Substance ID:
NACRES:
NA.21
Recommended Products
biological source
synthetic
Quality Level
grade
laboratory grade
description
non-ionic
form
liquid
mol wt
micellar avg mol wt 80,000
average mol wt 625
aggregation number
100-155
CMC
0.2-0.9 mM (20-25°C)
mp
6 °C (43 °F)
transition temp
cloud point 65 °C
pour point ~7 °C
solubility
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB
13.5
SMILES string
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key
HNLXNOZHXNSSPN-UHFFFAOYSA-N
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General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[3][4]
Application
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Features and Benefits
- Ideal for Cell Biology and Biochemical research applications
- Non-ionic nondenaturing detergent
- Superior wetting agent and emulsifier
- Highly versatile surfactant
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
483.8 °F - closed cup
Flash Point(C)
251 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XIV (Authorisation List)
CAS No.
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Articles
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
Protocols
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
This page shows how to solubilize membrane proteins with products from Cytiva.
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What's the molar concentration of the TritonX 100?? How can I make a 50mM concentration from this??
1 answer-
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What is the molecular weight of X100, Triton® X-100?
1 answer-
Based on an average of 9-10 ethylene oxide units per molecule, the molecular weight of Triton X-100 is 625 g/mol.
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Can I autoclave X100, Triton® X-100?
1 answer-
Aqueous solutions of Triton X-100 are stable to autoclaving. At certain concentrations the solutions may be cloudy but dispersible above the cloud point. However, the solution should clear with stirring upon cooling.
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Does X100, Triton® X-100, have any UV absorbance?
1 answer-
X100 does have UV absorbance at 280 nm, which will interfere with the UV determination of proteins.
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What is the concentration of X100, Triton® X-100?
1 answer-
This product is 100% active ingredient.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What concentration of X100,Triton® X-100, is typically used to lyse cells?
1 answer-
Typically, a 0.1 % solution of Triton X-100 is sufficient for lysing cells.
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