MAB2510
Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2
clone 264-449-2, Chemicon®, from mouse
Synonym(s):
GFP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
264-449-2, monoclonal
species reactivity (predicted by homology)
all
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Specificity
Directed against a bacterially-expressed fusion protein. Also reacts with red-shifted GFP-variant. Does not react with enhanced GFP.
Immunogen
Epitope: original GFP only
highly-purified native GFP
Application
Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2 is an antibody against Green Fluorescent Protein for use in IP, WB & IC.
Immunocytochemistry
Immunoblotting: 1:1000
Immunoprecipitation
Optimal working dilutions and protocols must be determined by the end user.
Immunoblotting: 1:1000
Immunoprecipitation
Optimal working dilutions and protocols must be determined by the end user.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Quality
Tested
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Storage and Stability
Store at 2-8°C for up to 12 months.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Alpha-synuclein-induced aggregation of cytoplasmic vesicles in Saccharomyces cerevisiae.
Soper, JH; Roy, S; Stieber, A; Lee, E; Wilson, RB; Trojanowski, JQ; Burd, CG; Lee, VM
Molecular Biology of the Cell null
Daniel Rodríguez-Martínez et al.
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Cryopreservation protocols are essential for stem cells storage in order to apply them in the clinic. Here we describe a new standardized cryopreservation protocol for GABAergic neural precursors derived from the medial glanglionic eminence (MGE), a promising source of GABAergic
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