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EZGRT

Millipore

Human Ghrelin ELISA Kit

measures and quantify total Ghrelin levels in 20 μL serum or plasma

Synonym(s):

Appetite Regulating Hormone, Growth hormone-releasing peptide, Motilin-related peptide, Protein M-46

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Human Ghrelin (total) ELISA, This Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.

Quality Level

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

20 μL sample volume (4hr assay)

assay range

accuracy: 101-107%
(Serum: Des-octanoyl Ghrelin)
accuracy: 88-91%
(Serum: Active Ghrelin)
linearity: 95-117%
(plasma)
linearity: 95.7-104.7%
(serum)
sensitivity: 156 pg/mL
(20 µL Sample Size)
inter-assay cv: 5.2-7.7%
intra-assay cv: 1.1-1.9%
(Plasma)
inter-assay cv: 6.2-7.8%
intra-assay cv: 0.9-1.3%
(Serum)

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum

NCBI accession no.

NM_016362.2

UniProt accession no.

Q9UBU3

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

General description

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach.

Specificity

Human Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)

Application

Research Category
Metabolism
Research Sub Category
Obesity

Metabolic Disorders
Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Used to detect/quantify: Ghrelin (Total)

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alteration in chromogranin A, obestatin and total ghrelin levels of saliva and serum in epilepsy cases.
Ersel Dag,Suleyman Aydin,Yusuf Ozkan,Fazilet Erman,Adile Ferda Dagli,Mehtap Gurger
Peptides null
The fermentable fibre inulin increases postprandial serum short-chain fatty acids and reduces free-fatty acids and ghrelin in healthy subjects.
Tarini J, Wolever TM
Applied Physiology, Nutrition, and Metabolism = Physiologie appliquee, Nutrition et metabolisme null
Immunohistochemical and quantitative analysis of ghrelin in Syzygium aromaticum.
Aydin S, Dagli AF, Ozkan Y, Kendir Y, Sahin I, Aksoy A, Ozercan IH.
Cell Biology International null
Y Falkén et al.
The Journal of clinical endocrinology and metabolism, 96(7), 2227-2235 (2011-05-06)
Endocrine effects of gastric bypass (GBP) surgery for obesity on glucose homeostasis are not fully understood. The main objective of the study was to assess the changes in plasma glucose, insulin, glucagon-like peptide-1 (GLP-1), leptin, somatostatin, glucose-dependent insulinotropic peptide, enteroglucagon

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