CBL419
Anti-Eosinophil Major Basic Protein Antibody, clone BMK13
clone BMK13, Chemicon®, from mouse
Synonym(s):
EMBP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
BMK13, monoclonal
species reactivity
human
should not react with
horse, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PRG2(5553)
Specificity
CBL419 is specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granual. It does not cross-react with horse, mouse or rat MBP.
Application
Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 is an antibody against Eosinophil Major Basic Protein for use in ELISA, WB, IH(P).
Immunohistochemistry: Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6μm), dried for 1 hour at room temperature and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr) should be used for skin biopsies. 5 μM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.
Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
ELISA development for MBP estimation.
Optimal working dilutions must be determined by the end user.
Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
ELISA development for MBP estimation.
Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Physical form
Format: Purified
The monoclonal is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage and Stability
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Toru Sonoyama et al.
International journal of molecular sciences, 23(21) (2022-11-12)
Transglutaminase (TGM) isoform catalyze the cross-linking reaction of identical or different substrate proteins. Eosinophil has been recognized in chronic rhinosinusitis with nasal polyps (CRSwNP) forming tissue eosinophil in nasal polyp (NP), and TGM isoforms are suggested to be associated with
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Hironobu Nishijima et al.
Laboratory investigation; a journal of technical methods and pathology, 96(9), 981-993 (2016-06-21)
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Ulcerative colitis and Crohn's disease are chronic inflammatory intestinal diseases with perplexing heterogeneity in disease manifestation and response to treatment. While the molecular basis for this heterogeneity remains uncharacterized, single-cell technologies allow us to explore the transcriptional states within tissues
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