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1.05449

Millipore

Wort broth (base)

GranuCult®, suitable for microbiology, for yeasts, for molds

Synonym(s):

Selective media for yeasts and molds, Yeasts and molds medium

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

form

medium granules (dehydrated (DCM))

Quality Level

200

packaging

pkg of 5 kg
pkg of 500 g

manufacturer/tradename

GranuCult®

storage condition

protect from light (Tightly closed. Dry)

technique(s)

microbiological culture: suitable

color

beige

pH

5.0 (30 °C, 33 g/L in H2O, after autoclaving)

solubility

33 g/L

bulk density

650 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

molds
yeasts

General description

Wort broth (base) is used for the cultivation, isolation, and enumeration or enrichment of yeasts. The addition of certain indicator dyes to the Wort broth base allows differentiation between yeast and bacterial colonies. The accompanying bacterial flora is weakly suppressed by the pH value of 5.0 and largely by a pH of 3.5.

Application

Wort broth (base) is suitable for the cultivation, isolation, and enumeration or enrichment of fungi, especially yeasts.

Features and Benefits

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 4.8 - 5.2
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good/very good
Growth (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): good/very good
Growth (Saccharomyces cerevisiae ATCC 9080): good/very good
Growth (Geotrichum candidum DSM 1240): good/very goog
Growth (Rhodotorula mucilaginosa DSM 70403): good/very good
Growth (Penicillium commune ATCC 10428): good/very good
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): good/very good
Growth (Trichophyton ajelloi ATCC 28454): good/very good
Incubation: 7 days; 28 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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