T7659
Trypsin Inhibitor, Defined (1X) Solution
Animal component free, BioReagent, suitable for cell culture
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About This Item
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sterility
sterile-filtered
product line
BioReagent
form
solution
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
Application
This product designed to be used during the subculture of keratinocytes contains Kunitz-type soybean trypsin inhibitor (SBTI) which inhibits the catalytic activity of serine proteases such as trypsin and tryptase. It is used in cell culture to stop the action of trypsin which is used to release cells from substratum during passaging. It may be also useful in other anti-serine protease studies.
Used to neutralize the effects of trypsin/EDTA solution.
Physical form
Solution in 98.7% DPBS and 1.3% Soybean Trypsin Inhibitor.
Reconstitution
Use at a minimum volume ratio of 1:1 TID:trypsin/EDTA.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.
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