HPLC Analysis of Tamoxifen E/Z isomers and Related Compounds on Ascentis^® Express RP-Amide

column

Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 μm particles (53918-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile

gradient

held at 25% B for 2 min; 25 to 40% B in 15 min; 40 to 25% B in 0.1 min; held at 25% B for 2.9 min

flow rate

0.6 mL/min

pressure

2292 psi (158 bar)

column temp.

35 °C

detector

UV, 260 nm

injection

2 μL

sample

10 mg/L in 10:90, water: methanol