Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

MAK089

Sigma-Aldrich

γ-Glutamyltransferase (GGT) Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

GGT Colorimetric Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... GGT1(2678) , GGT2(728441) , GGT5(2687) , GGT6(124975) , GGT7(2686)
mouse ... GGT1(14598) , GGT5(23887) , GGT6(71522) , GGT7(207182)
rat ... GGT1(116568) , GGT5(29566) , GGT6(408206) , GGT7(156275)

General description

γ-Glutamyltransferase (GGT, EC 2.3.2.2) is a membrane-bound protein that catalyzes the transfer of -glutamyl moieties to acceptor molecules such as amino acids or peptides. GGT plays a key role in the λ-glutamyl cycle, a critical pathway for glutathione homeostasis as well as the detoxification of xenobiotics. Serum GGT levels can be elevated in many pathophysiological conditions such as cardiovascular disease, chronic liver disease, and metabolic syndrome. Elevated serum levels of GGT can also be indicative of oxidative stress.

Application

γ-Glutamyltransferase (GGT) Activity Colorimetric Assay Kit has been used to assess the activity of γ-glutamyltransferase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of gamma-glutamyl transferase (GGT) in a variety of samples including tissue, cells and serum

Principle

The GGT Activity Colorimetric Assay kit provides a simple and direct procedure for measuring GGT activity in a variety of samples. GGT activity is determined by a coupled enzyme assay, in which the GGT transfers the γ-glutamyl group from the substrate L-γ-Glutamyl-p-nitroanilide, liberating the chromogen p-nitroanilide (pNA, 418 nm) proportional to the GGT present. One unit of GGT is the amount of enzyme that will generate 1.0μmole of pNA per minute at 37 °C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Eslam E Abd El-Fattah et al.
Frontiers in pharmacology, 12, 720173-720173 (2022-02-01)
HIF-1α is a key factor promoting the development of hepatocellular carcinoma (HCC). As well, AKT-AMPKα-mTOR signaling is a promising target for cancer therapy. Yet, the AKT-AMPKα-mTOR-dependent activation of HIF-1α has not been studied in livers with HCC. In addition, the
Floris J M Roos et al.
Clinical and translational medicine, 11(12), e566-e566 (2021-12-27)
The well-established 3D organoid culture method enabled efficient expansion of cholangiocyte-like cells from intrahepatic (IHBD) and extrahepatic bile duct (EHBD) tissue biopsies. The extensive expansion capacity of these organoids enables various applications, from cholangiocyte disease modelling to bile duct tissue
Floris J M Roos et al.
Frontiers in cell and developmental biology, 8, 630492-630492 (2021-02-27)
Diseases of the bile duct (cholangiopathies) remain a common indication for liver transplantation, while little progress has been made over the last decade in understanding the underlying pathophysiology. This is largely due to lack of proper in vitro model systems
Tribbles-1 regulates hepatic lipogenesis through posttranscriptional regulation of C/EBPα.
Bauer R C, et al.
The Journal of Clinical Investigation, 125(10), 3809-3818 (2015)
Robert C Bauer et al.
The Journal of clinical investigation, 125(10), 3809-3818 (2015-09-09)
Variants near the gene TRIB1 are significantly associated with several plasma lipid traits, circulating liver enzymes, and the development of coronary artery disease in humans; however, it is not clear how its protein product tribbles-1 regulates lipid metabolism. Here, we
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service