L8005

β-Lactoglobulin B from bovine milk

≥90% (PAGE)

• Synonym(s): β-LG, Bos d 5, beta-LG
• CAS Number: 9066-45-9
• MDL number: MFCD00130637
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine milk
• Assay: ≥90% (PAGE)
• form: powder
• mol wt: 18,276 Da by calculation
• technique(s): HPLC: suitable; electrophoresis: suitable
• UniProt accession no.: P02754
• storage temp.: 2-8°C
• Gene Information: bovine ... LGB(280838)

Description

General description

β-Lactoglobulin B (β-LG B), a isoform of β-Lactoglobulin, is a small protein of 162 amino acids with a molecular mass of 18.2 kDa and optimum pH of 5.2. It is present in high level in casein and is relatively low in raw bovine milk. β-LG has eight-stranded β-barrel (strands A-H) succeeded by a three-turn α-helix and a final β-strand (strand I), that forms part of the dimerization interface.

Milk from dairy cows contains the protein β-lactoglobulin (BLG). It naturally occurs in a number of genetic variants, and the most prevalent bovine variants are known as BLG A and BLG B.

Application

β-Lactoglobulin B from bovine milk has been used as:

• a protein standard in SDS-polyacrylamide electrophoresis for quantification of milk protein fractions
• in the calibration of reversed phase- high-performance liquid chromatography (HPLC) for caseins quantification
• for immobilization on the biosensor surface and a calibration standards in biosensor assay

β-Lactoglobulin was used in the identification of the genetic variants of κ-casein in milk by isoelectric focusing electrophoresis.

Biochem/physiol Actions

β-Lactoglobulin B (β-LG B) show less inhibitory effect on the Staphylaococcus sp compared to β-LG A.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves