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11873601001

Roche

Pefabloc® SC PLUS

crystalline, suitable for blocking, suitable for protein purification, 0.1-1.0 mg/mL

Synonym(s):

pefabloc, serine protease inhibitor

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About This Item

UNSPSC Code:
12352204

form

crystalline

Quality Level

mol wt

239.5

packaging

pkg of 5 mL (100 mg)

manufacturer/tradename

Roche

concentration

0.1-1.0 mg/mL

technique(s)

blocking: suitable
protein purification: suitable

optimum pH

neutral

mp

175-185 °C

solubility

soluble (Solubility and stability of the protease inhibitor remains unchanged.)

storage temp.

2-8°C

General description

Pefabloc SC is a specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity is comparable to PMSF or DFP, but Pefabloc SC is nontoxic to cells. The Pefabloc SC PLUS set combines the serine protease inhibitor Pefabloc SC with a uniquely formulated Pefabloc SC protector (PSC-Protector), adding additional convenience and reliability to the safe, stable protease inhibition provided by Pefabloc SC.

Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH.

Specificity

Pefabloc SC belongs to the family of sulfonyl fluorides which irreversibly block serine proteases. Pefabloc was also described as potent serine threonine phosphatase inhibitor.
Specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity of Pefabloc SC is comparable to PMSF or DFP; however, Pefabloc SC is non-toxic. Specifically inhibits serine proteases and prevents nonspecific covalent modification of proteins as tested by mass spectrometry.

Application

  • Pefabloc® SC is used to inhibit the detrimental effects of proteases during preparative protein purification. The PSC-protector solution prevents nonspecific covalent modification of proteins when Pefabloc is used in higher concentrations and extended incubation times at an alkaline pH (tested by mass spectrometry).
  • Due to its low toxicity toward eukaryotic cells, it may be applied in the production of recombinant proteins, during fermentation of transformed cells, where proteolytic digestion may decrease the yield of the desired product.
  • Pefabloc SC is used to completely inactivate proteinase K during the preparation of chromosomal DNA in agarose plugs. For this purpose, the cells are embedded in agarose and digested with proteinase K to degrade all proteins. Before a specific restriction endonuclease is added, the proteinase K is inactivated by incubating the agarose plugs in 1 – 5 mM Pefabloc SC in 10 mM Tris-HCl, 1 mM EDTA , pH 7 for 2 hours at +37 °C or overnight.
  • In contrast to PMSF, Pefabloc SC is an excellent blocker of thrombin activity in serum or plasma. In these biological fluids, PMSF interacts in a reversible manner with albumin, which reduces its free concentration and leads to a delay in thrombin inactivation. Pefabloc SC, however, does not react with serum albumin, and exhibits a threefold higher capacity to inactivate thrombin under similar conditions.
  • Pefabloc SC can be used on living cells.
Pefabloc® SC PLUS has been used in digitonin extraction buffer and in cathepsin B activity assay buffer in THP-1 cells. It has also been used as a component of iodixanol solution and sucrose buffer for the isolation of nuclei from Xenopus embryos.

Features and Benefits

  • Take advantage of a simplified two-reagent system. Use the PSC-Protector solution to prevent covalent binding between proteins and Pefabloc SC, even at high concentrations, extended incubation times, and at alkaline pH.
  • Benefit from an easy-to-use inhibitor. Add water-soluble Pefabloc SC directly to aqueous buffers.
  • Avoid hazardous compounds. Obtain non-toxic protease inhibition without risk to you, or those around you.
  • Ensure protection with improved stability. Achieve consistent protease inhibition even at pH levels above 7.0 and temperatures above +4°C.
  • Maximize inhibition. Be certain that your levels of active inhibitor are high enough by using a product that is reliably soluble and stable.

Packaging

Set containing two components

Preparation Note

Working concentration: 0.1 - 1.0 mg/ml (0.4 - 4 mM)
Pefabloc SC plus the corresponding amount of PSC-protector
Working solution: Recommended solvent is distilled water or aqueous buffer (for Pefabloc SC).
The solubility is up to 0.1 M for Pefabloc SC.
Storage conditions (working solution): -15 to -25 °C (stock solution)
Solubility and stability of the protease inhibitor remains unchanged. See Pefabloc SC.

Reconstitution

Solubility and stability of the protease inhibitor remains unchanged. See Pefabloc SC.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Pefabloc is a registered trademark of Pentapharm

Kit Components Only

Product No.
Description

  • Pefabloc SC, 100 mg

  • PSC-Protector solution, 5 mL ready-to-use

Pictograms

FlameCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 1


Certificates of Analysis (COA)

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Chikodili C Nduaguibe et al.
Islets, 2(3), 200-206 (2010-11-26)
Pefabloc, Trasylol and Urinary Trypsin Inhibitor (UTI) have been reported to be effective serine protease inhibitors that impair pancreatic endogenous proteases resulting in improved islet yield. Here we evaluated the effect of these inhibitors on endogenous proteases (trypsin, chymotrypsin and
Vikte Lionikaite et al.
Journal of leukocyte biology, 104(6), 1133-1145 (2018-07-31)
Increased intake of vitamin A (retinoids) is associated with decreased bone mass and increased fracture risk in humans. Mechanistic studies in rodents have shown that hypervitaminosis A results in decreased bone mass caused by an increase in periosteal osteoclasts while
Yonggang Zhang et al.
Oncotarget, 6(8), 6160-6178 (2015-02-24)
Regulatory mechanisms underlying constitutive and inducible NFκB activation in cancer remain largely unknown. Here we investigated whether a novel NIK- and IKK2-binding protein (NIBP) is required for maintaining malignancy of cancer cells in an NFκB-dependent manner. Real-time polymerase chain reaction
DNase-seq to study chromatin accessibility in early Xenopus tropicalis embryos
Cho JS, et al.
Cold Spring Harbor Protocols, 4 (2019)
Aaron J Ogden et al.
International journal of molecular sciences, 21(12) (2020-06-27)
Drought is the leading cause of agricultural yield loss among all abiotic stresses, and the link between water deficit and phloem protein contents is relatively unexplored. Here we collected phloem exudates from Solanum lycopersicum leaves during periods of drought stress

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