P3288

Phosphate buffer pH 7.2 at 25 °C

for hematology and histology staining techniques

• Synonym(s): phosphate buffer
• NACRES: NA.47
• UNSPSC Code: 12352107

Properties

• Quality Segment: 200
• form: powder
• color: white
• pH: 7.10-7.30
• application(s): hematology; histology
• storage temp.: room temp

Description

General description

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).

Application

Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.

Preparation Note

Each vial will prepare 3.8 L of buffer, 8.3 mM.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable