MTOXRCSUP

RPTEC Complete Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

• Synonym(s): Basal media supplement for huRPTEC
• UNSPSC Code: 12352207
• NACRES: NA.81

Properties

• Quality Level: 300
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• storage temp.: −20°C

Description

General description

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Application

RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.

Preparation Note

Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO₂ in air atmosphere.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable