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M4528

Sigma-Aldrich

Monoclonal Anti-MAP1b antibody produced in mouse

clone AA6, ascites fluid

Synonym(s):

Anti-MAP1.2, Anti-MAP1X, Anti-MAP5

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

AA6, monoclonal

contains

15 mM sodium azide

species reactivity

human, feline, bovine, hamster, chicken, mouse, rat

technique(s)

microarray: suitable
western blot: 1:500 using a fresh total rat brain extract or an enriched microtubule protein preparation

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP1B(4131)
mouse ... Mtap1b(17755)
rat ... Map1b(29456)

General description

MAP1b is a microtubule associated protein that interacts with actin filaments. MAP1b regulates microtubule stabilization and the development of axons. It may also act as a scaffolding protein Addition of antibody to microtubule proteins before polymerization in immunoassays results in abnormally short (but otherwise morphologically normal) microtubules. Immunohistochemical staining of brain tissue with the product shows selective labeling of dendritic trees throughout the brain. Monoclonal Anti-MAP1b does not react with tubulin of other microtubule associated proteins.
Monoclonal Anti-MAP1b (MAP5) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Microtubule-associated protein (MAP1B) is the major microtubule associated protein in developing brain which changes its expression during development. In the new born rat brain, it is a major component of microtubules but in the adult its level is ten-fold lower.

Specificity

Monoclonal Anti-MAP1b antibody is specific for MAP1b in humans, mice, rats, bovines, cats, hamsters and chickens. The antibody does not cross-react with other MAPs or tubulin. Addition of the antibody to microtubule proteins before polymerization results in abnormally short (but otherwise morphologically normal) microtubules. In immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons, dendrites and cell bodies.

Immunogen

rat brain microtubule-associated proteins (MAPs)

Application

Monoclonal Anti-MAP1b antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunostaining
  • fluorescence microscopy

Biochem/physiol Actions

Microtubule-associated protein (MAP1B) is the first MAP to appear in growing axons during development as it is present from the first emergence of the nascent axon from the cell body. Monoclonal Anti-MAP1b may be used to study MAP expression and cytological localization both in tissues and cell lines, under different developmental and environmental circumstances.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Masamitsu Shimazawa et al.
The Journal of pharmacology and experimental therapeutics, 332(2), 380-387 (2009-11-17)
We examined whether ((1S)-1-((((1S)-1-benzyl-3-cyclopropylamino-2,3-di-oxopropyl)amino)carbonyl)-3-methylbutyl)carbamic acid 5-methoxy-3-oxapentyl ester (SNJ-1945), a new orally available calpain inhibitor, might reduce retinal cell death in vivo and/or in vitro. Retinal cell damage was induced in vivo in mice by intravitreal injection of N-methyl-d-aspartate (NMDA), and
Human mesenchymal precursor cells (Stro-1+) from spinal cord injury patients improve functional recovery and tissue sparing in an acute spinal cord injury rat model
Hodgetts SI, et al.
Cell Transplantation, 22(3), 393-412 (2013)
Association of microtubule-associated protein (MAP1B) with growing axons in cultured hippocampal neurons
Fischer I and Romano CG
Molecular and Cellular Neurosciences, 2(1), 39-51 (1991)
Carolina Montenegro-Venegas et al.
Molecular biology of the cell, 21(20), 3518-3528 (2010-08-20)
Cultured neurons obtained from MAP1B-deficient mice have a delay in axon outgrowth and a reduced rate of axonal elongation compared with neurons from wild-type mice. Here we show that MAP1B deficiency results in a significant decrease in Rac1 and cdc42
Human pluripotent stem cells and derived neuroprogenitors display differential degrees of susceptibility to BH3 mimetics ABT-263, WEHI-539 and ABT-199
Garcia CP, et al.
Testing, 11(3), e0152607-e0152607 (2016)

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