AB5032
Anti-Neural Cell Adhesion Molecule Antibody
Chemicon®, from rabbit
Synonym(s):
CD56 antigen, antigen recognized by monoclonal antibody 5.1H11
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, chicken, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NCAM1(4684)
General description
The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
The previously assigned protein identifier P13592 has been merged into P13591. Full details can be found on the UniProt database.
Specificity
Expected to cross-react with Chicken, Human, and rat.
Neural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Immunogen
Highly purified chicken NCAM
Application
Detect Neural Cell Adhesion Molecule using this Anti-Neural Cell Adhesion Molecule Antibody validated for use in ELISA, IF, IH, FUNC & WB.
Immunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Function blocking:
A previous lot of this antibody was used in Function blocking.
ELISA:
0.3 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Function blocking:
A previous lot of this antibody was used in Function blocking.
ELISA:
0.3 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Growth Cones & Axon Guidance
Quality
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
Target description
MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa A breakdown product of 160 kDa may also be visible.
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS containing no preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Neuraminidase-treated mouse brain membrane.
Neuraminidase-treated mouse brain membrane.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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