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Molecular Choreography of Acute Exercise.

Cell (2020-05-30)
Kévin Contrepois, Si Wu, Kegan J Moneghetti, Daniel Hornburg, Sara Ahadi, Ming-Shian Tsai, Ahmed A Metwally, Eric Wei, Brittany Lee-McMullen, Jeniffer V Quijada, Songjie Chen, Jeffrey W Christle, Mathew Ellenberger, Brunilda Balliu, Shalina Taylor, Matthew G Durrant, David A Knowles, Hani Choudhry, Melanie Ashland, Amir Bahmani, Brooke Enslen, Myriam Amsallem, Yukari Kobayashi, Monika Avina, Dalia Perelman, Sophia Miryam Schüssler-Fiorenza Rose, Wenyu Zhou, Euan A Ashley, Stephen B Montgomery, Hassan Chaib, Francois Haddad, Michael P Snyder
ABSTRACT

Acute physical activity leads to several changes in metabolic, cardiovascular, and immune pathways. Although studies have examined selected changes in these pathways, the system-wide molecular response to an acute bout of exercise has not been fully characterized. We performed longitudinal multi-omic profiling of plasma and peripheral blood mononuclear cells including metabolome, lipidome, immunome, proteome, and transcriptome from 36 well-characterized volunteers, before and after a controlled bout of symptom-limited exercise. Time-series analysis revealed thousands of molecular changes and an orchestrated choreography of biological processes involving energy metabolism, oxidative stress, inflammation, tissue repair, and growth factor response, as well as regulatory pathways. Most of these processes were dampened and some were reversed in insulin-resistant participants. Finally, we discovered biological pathways involved in cardiopulmonary exercise response and developed prediction models revealing potential resting blood-based biomarkers of peak oxygen consumption.

MATERIALS
Product Number
Brand
Product Description

Millipore
MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 1 - Cardiovascular Disease Multiplex Assay, The analytes available for this multiplex kit are: BNP, NT-proBNP, CK-MB, CXCL6/GCP-2, CXCL16, Endocan-1 (ESM-1), FABP3, FABP4, LIGHT, Oncostatin (OSM), Placental Growth Factor (PlGF), Troponin I (TnI).
Millipore
MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay, The analytes available for this multiplex kit are: ADAMTS13, D-Dimer, GDF-15, Myoglobin, sICAM-1, MPO, P-selectin, Lipocalin-2/NGAL, sVCAM-1, SAA.
Millipore
MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 4 - Cardiovascular Disease Multiplex Assay, The analytes available for this multiplex kit are: sE-Selectin, Follistatin, Pecam-1, Pentraxin-3, Tissue Factor (TF), Thrombomodulin, Troponin T (TnT).
Millipore
MILLIPLEX® Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay, The Human Metabolic Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 13 protein biomarkers in human serum, plasma and cell/tissue culture samples.
Millipore
MILLIPLEX® Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 - Cardiovascular Disease Multiplex Assay, The analytes available for this multiplex kit are: Adipsin, AGP, α2-Macroglobulin, CRP, Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P, Haptoglobin, & Platelet Factor-4.