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  • Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells.

Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells.

European journal of histochemistry : EJH (2009-06-29)
Y H Huang, M J Sun, M Jiang, B Y Fu
ABSTRACT

We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Donkey Anti-Sheep IgG Antibody, FITC conjugate, Species Adsorbed, Chemicon®, from donkey
Sigma-Aldrich
Donkey Anti-Guinea Pig IgG Antibody, Cy3 conjugate, Species Adsorbed, Chemicon®, from donkey