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RAB0312

Sigma-Aldrich

Rat Interleukin-6 ELISA Kit

for for cell lysate and tissue lysate

Synonym(s):

IL-6, Interleukin-6 

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 40.96-10000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Il6(24498)

General description

The Rat IL-6 ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-6 cell lysate and tissue lysate.

Immunogen

Recombinant Rat IL-6

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Rat Interleukin-6 ELISA Kit has been used to determine the plasma level of interleukin-6 (IL-6) via ELISA.

Biochem/physiol Actions

Interleukin-6 (IL-6) acts as a neuromodulation factor. IL-6 has two signalling pathways, known as classic signalling pathway and trans-signalling pathway. It has a neuroprotective role in ischemic stroke, by activating IL-6/interleukin 6 receptor (IL-6R)/signal transducers and activators of transcription 3 (STAT3) signalling pathway. IL-6 is a multifunctional cytokine, which is implicated in acute inflammation. IL-6 is associated with SAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABLYSIS12X Cell Lysis Buffer (Item J)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Serum IL-6 and IL-23 levels and their correlation with angiogenic cytokines and disease activity in ankylosing spondylitis, psoriatic arthritis, and SAPHO syndrome
Przepiera-Bkedzak H, et al.
Mediators of Inflammation, 2015, 2529-2537 (2015)
Lars Wallentin et al.
European heart journal, 41(41), 4037-4046 (2020-09-29)
The global COVID-19 pandemic is caused by the SARS-CoV-2 virus entering human cells using angiotensin-converting enzyme 2 (ACE2) as a cell surface receptor. ACE2 is shed to the circulation, and a higher plasma level of soluble ACE2 (sACE2) might reflect
Thomas A Rasmussen et al.
The lancet. HIV, 5(5), e221-e230 (2018-04-13)
Whether ongoing virus replication occurs in HIV-infected individuals on antiretroviral therapy (ART) is unclear; therefore, whether residual virus replication is a barrier to achieving a cure for HIV is also unknown. We aimed to establish whether ART intensification with dolutegravir
Celestino Sardu et al.
Journal of the American Heart Association, 9(17), e016948-e016948 (2020-07-08)
BACKGROUND Coronavirus disease 2019 (COVID-19) is the cause of a pandemic disease, with severe acute respiratory syndrome by binding target epithelial lung cells through angiotensin-converting enzyme 2 in humans. Thus, patients with hypertension with COVID-19 could have worse prognosis. Indeed
Mike Recher et al.
NPJ vaccines, 3, 17-17 (2018-05-26)
Pneumococcal conjugate vaccine (PCV) is recommended for adults with chronic disease. Extensive limb swelling (ELS) is an acute vigorous local inflammatory reaction following vaccination. Predisposing immune system correlates and the influence of ELS on vaccine responses are not known. Here

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