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G9768

Sigma-Aldrich

Guanosine 5′-triphosphate–Agarose

aqueous glycerol suspension

Synonym(s):

5′-GTP agarose

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About This Item

MDL number:
MFCD00171655
UNSPSC Code:
41106500
NACRES:
NA.56

biological source

bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast

form

aqueous glycerol suspension

extent of labeling

1-5 μmol per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

ribose hydroxyls

matrix spacer

11 atoms (adipic acid dihydrazide)

storage temp.

−20°C

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Application

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

Physical form

Guanosine 5′-triphosphate–Agarose has been used:
  • in chromatographic column for the purification of a translocation stimulating factor
  • in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)
  • in GTP-Binding assay
  • for the depletion of GTP binding proteins in cytosol of rat liver
Suspension in 50% glycerol containing 0.25 M NaCl

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificates of Analysis (COA)

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T E Allen et al.
Molecular and biochemical parasitology, 65(2), 233-245 (1994-06-01)
The hypoxanthine-guanine phosphoribosyltransferase (HGPRT) enzyme in Trypanosoma cruzi is a rational target for the treatment of Chagas disease. To evaluate the T. cruzi HGPRT in detail, the HGPRT gene (hgprt) was cloned from a genomic library of T. cruzi DNA
Possible role of both the alpha and beta gamma subunits of the heterotrimeric G protein, Gs, in transcytosis of the polymeric immunoglobulin receptor
Bomsel M and Mostov KE
The Journal of Biological Chemistry, 268, 25824-25835 (1993)
70K heat shock related proteins stimulate protein translocation into microsomes
Chirico WJ, et al.
Nature, 332, 805-805 (1988)
Karl J Mathis et al.
Protein expression and purification, 60(1), 58-63 (2008-04-24)
Soluble guanylate cyclase (sGC), the main target of nitric oxide (NO), is a cytosolic, heme-containing, heterodimeric enzyme that catalyzes the conversion of guanosine 5'-triphosphate (GTP) to 3,5'-cyclic guanosine monophosphate (cGMP) and pyrophosphate (PPi) in the presence of Mg2+. Cyclic GMP
Jong Hyun Kim et al.
Nature communications, 8(1), 732-732 (2017-10-01)
Leucyl-tRNA synthetase (LRS) is known to function as leucine sensor in the mammalian target of rapamycin complex 1 (mTORC1) pathway. However, the pathophysiological significance of its activity is not well understood. Here, we demonstrate that the leucine sensor function for mTORC1
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