HCVD6MAG-67K
MILLIPLEX® Human Cardiovascular Disease Magnetic Bead Panel 6 - Cardiovascular Disease (CVD) Multiplex Assay
The analytes available for this multiplex kit are: sCD14, DPP4/sCD26, Endostatin, pGRN, LRG1, MCAM, ucMGP
About This Item
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 92%
(Endostatin)
accuracy: 95%
(ucMGP)
accuracy: 97%
(DPP4/sCD26)
sensitivity: 0.007 ng/mL
(MinDC+2SD; Endostatin)
sensitivity: 0.014 ng/mL
(MinDC+2SD; pGRN)
sensitivity: 0.015 ng/mL
(MinDC+2SD; LRG1)
sensitivity: 0.016 ng/mL
(MinDC+2SD; MCAM)
sensitivity: 0.016 ng/mL
(MinDC+2SD; sCD14)
sensitivity: 0.041 ng/mL
(MinDC+2SD; DPP4)
sensitivity: 0.052 ng/mL
(MinDC+2SD; ucMGP)
standard curve range: 0.002-10 ng/mL
(Endostatin)
standard curve range: 0.01-40 ng/mL
(sCD14)
standard curve range: 0.024-100 ng/mL
(LRG1)
standard curve range: 0.024-100 ng/mL
(MCAM)
standard curve range: 0.024-100 ng/mL
(pGRN)
standard curve range: 0.049-200 ng/mL
(DPP4/sCD26)
standard curve range: 0.049-200 ng/mL
(ucMGP)
inter-assay cv: <10%
intra-assay cv: <5%
(DPP4/sCD26)
inter-assay cv: <10%
intra-assay cv: <5%
(Endostatin)
inter-assay cv: <10%
intra-assay cv: <5%
(LRG1)
inter-assay cv: <10%
intra-assay cv: <5%
(MCAM)
inter-assay cv: <10%
intra-assay cv: <5%
(pGRN)
inter-assay cv: <20%
intra-assay cv: <10%
(ucMGP)
inter-assay cv: <20%
intra-assay cv: <5%
(sCD14)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
ambient
General description
The MILLIPLEX® Human Cardiovascular Disease (CVD) Panel 6 is a 7-plex kit may be used to simultaneously quantify any or all the following analytes in human serum, plasma, and tissue culture samples: Soluble Cluster of Differentiation 14 (sCD14), Dipeptidyl Peptidase-4 (DPP4/DPPIV) / Soluble Cluster of Differentiation 26 (sCD26), Endostatin / Collagen Alpha-1 (XVIII) (COL181A), Progranulin (pGRN) / Proepithelin (PEPI) / PC Cell-derived Growth Factor (PCDGF) / Acrogranin / Paragranulin, Leucine-rich Alpha-2-Glycoprotein 1 (LRG1), Melanoma Cell Adhesion Molecule (MCAM) / Soluble Cluster of Differentiation 146 (sCD146) / Cell Surface Glycoprotein MUC18, Uncarboxylated Matrix Gamma-Carboxyglutamate (Gla) Protein (ucMGP). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cardiovascular
Specificity
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Application
- Analytes: Monocyte Differentiation Antigen (CD14/sCD14), Dipeptidyl Peptidase-4 (DPP4/sCD26), Endostatin, Leucine-rich α-2-glycoprotein 1 (LRG1), Cell Surface Glycoprotein (MCAM/ MUC18/sCD146), Progranulin (pGRN), Uncarboxylated Matrix Gla Protein (ucMGP)
- Recommended Sample Type:
- Human serum, plasma, cell/tissue culture supernatants and lysates
- Recommended Sample Dilution: 25 μL per well of 1:500 diluted serum or plasma; cell/tissue culture supernatants or lysates may be used undiluted ore or diluted in appropriate control medium
- Assay Run Time: Overnight (16-18 hours)at 2-8°C will yield optimal sensitivity.2- A 2 hour incubation at room temperature (20-25°C) may also be used.
- Research Category: Cardiovascular Disease
- Research Subcategory: Metabolic Disorders
Features and Benefits
Packaging
Storage and Stability
Other Notes
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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