SAB4200274

Anti-EIF2C2/Ago2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Eukaryotic translation initiation factor 2C, 2, Anti-I2C2, Argonaute-2, Anti-PAZ Piwi domain protein, Anti-Slicer protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~97 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human EIF2C2/Ago2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EIF2C2(27161) ; mouse ... Eif2c2(239528) ; rat ... Eif2c2(59117)

Description

General description

Anti-eukaryotic translation initiation factor 2C 2 (EIF2C2)/protein argonaute-2 (Ago3) is produced in rabbit using as immunogen a synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2 conjugated to KLH. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of hsAgo1-4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies.

Immunogen

synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Ago2 has endonuclease activity, that cleaves the target RNA in RNAi. Ago2 is regulated at both the transcriptional and posttranslational levels in human breast cancer cell lines. It is implicated in micro-RNA activity and in the tumorigenic progression of breast cancer cell lines.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable