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S2640

Sigma-Aldrich

Serum Replacement 3 (50×)

liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Serum Substitute

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
sterility:
sterile-filtered
form:
liquid
Pricing and availability is not currently available.

sterility

sterile-filtered

Quality Level

200

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

General description

Sigma′s Serum Replacement 3 is a fortified, multipurpose serum replacement for the long-term culturing of anchorage dependant and suspension cultures. This serum replacement is effective in research situations such as: studying the effects of growth factors or in the manufacturing of cell derived products.

Application

Serum Replacement 3 (50×) has been used as a supplement in modified phosphate-buffered saline (DPBS)[1] and as a replacement for the growth medium for in vitro experiments for Tendon cells.[2]

Other Notes

Defined serum replacement designed primarily for the long-term growth of human cells or other mammalian cells. Recommended for use in the production of cell-secreted proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, human recombinant insulin). Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells.

Warning

This product is tested and found non-reactive for Hepatitis B Surface Antigen (HBsAg) and non-reactive for Human Immunodeficiency Virus (HIV) antibody by ELISA. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly.

Analysis Note

Stable for 30 days when diluted in cell culture medium.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCW1429
MKCX0121
MKCX0122
MKCT6952
MKCV3297

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Electrical properties of breast cancer cells from impedance measurement of cell suspensions
IEEE Transactions on Bio-Medical Engineering, 224, 012081-012081 (2010)
HGF Mediates the Anti-inflammatory Effects of PRP on Injured Tendons
Zhang J, et al.
PLoS ONE, 8(6), e67303-e67303 (2013)
Lea Bornemann et al.
BMC cancer, 20(1), 650-650 (2020-07-15)
The identification of pathologically altered neutrophil granulocyte migration patterns bears strong potential for surveillance and prognostic scoring of diseases. We recently identified a strong correlation between impaired neutrophil motility and the disease stage of myelodysplastic syndrome (MDS). Here, we apply
Eric J Suh et al.
Genome biology, 13(12), R121-R121 (2012-12-25)
Although quiescence (reversible cell cycle arrest) is a key part in the life history and fate of many mammalian cell types, the mechanisms of gene regulation in quiescent cells are poorly understood. We sought to clarify the role of microRNAs

Questions

1–2 of 2 Questions  

  1. What is the difference between Serum Replacement 1, 2, and 3?

    1 answer

    1. These concentrated serum replacements are defined, as multipurpose products designed to eliminate the need for serum in culture. These products do not contain growth factors, steroid hormones, or mitogens.

      S0638 Serum Replacement 1, 50X
      Total protein concentration at 1X of 770 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S9388 Serum Replacement 2, 50X
      Total protein concentration at 1X of 570 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S2640 Serum Replacement 3, 50X
      Total protein concentration at 1X of 664 mg/mL
      Highly purified human serum albumin (heat treated)
      Human transferrin (heat treated)
      Human (recombinant) insulin

      Helpful?

  2. Does the Serum Replacement 3 contain heat-treated bovine serum albumin, transferrin and insulin, like with Serum Replacement 1 and 2?

    1 answer

    1. As described in the "Other Notes" section of the product detail page, this is a defined serum replacement designed primarily for the long-term growth of human cells or other mammalian cells. Recommended for use in the production of cell-secreted proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, human recombinant insulin). Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells. It does not contain bovine proteins like products S0638, Serum Replacement 1 (50×) and S9388, Serum Replacement 2 (50×).

      Helpful?

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