Skip to Content
Merck
All Photos(3)

Documents

M2405

Sigma-Aldrich

4-Methylumbelliferyl β-D-lactopyranoside

β-galactosidase substrate, ≥98.00% (TLC), powder

Synonym(s):

4-Methylumbelliferyl β-D-lactoside

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C22H28O13
CAS Number:
84325-23-5
Molecular Weight:
500.45
Beilstein:
8094402
MDL number:
MFCD00083406
UNSPSC Code:
12352204
PubChem Substance ID:
24896730
NACRES:
NA.32

product name

4-Methylumbelliferyl β-D-lactopyranoside, β-galactosidase substrate

Quality Level

200

Assay

≥98.00% (TLC)

form

powder

solubility

water: 9.80-10.20 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 365 nm; λem 445 nm (after enzymatic degradation)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15+,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-KSFLKEQHSA-N

Looking for similar products? Visit Product Comparison Guide

Application

4-Methylumbelliferyl β-D-lactopyranoside has been used as a chromophoric substrate for cellobiohydrolase I (CBHI) in T. reesei. It has also been used as a substrate for cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase from fermented samples in enzymatic assay.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-lactopyranoside is a fluorescence compound hydrolyzed by the enzyme β-glucosidase (βG) and CBH1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

M J Bailey et al.
Applied microbiology and biotechnology, 62(2-3), 156-162 (2003-04-08)
A low-foaming hydrophobin II deletant of the Trichoderma reesei strain Rut-C30 was used for production of cellulases by continuous cultivation on lactose medium in a laboratory fermenter. The control paradigm of the addition of new medium to the continuous process
Ana Cristina Rodrigues et al.
Bioresource technology, 156, 163-169 (2014-02-08)
The potential of enzymes recycling after hydrolysis and fermentation of wheat straw under a variety of conditions was investigated, monitoring the activity of the enzymes in the solid and liquid fractions, using low molecular weight substrates. A significant amount of
Yaron Shav-Tal et al.
FEBS letters, 531(2), 109-114 (2002-11-06)
Proteins are often referred to in accordance with the activity with which they were first associated or the organelle in which they were initially identified. However, a variety of nuclear factors act in multiple molecular reactions occurring simultaneously within the
Noam Fallik et al.
Oncotarget, 8(65), 109575-109586 (2018-01-10)
Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs
Ana Cristina Rodrigues et al.
Enzyme and microbial technology, 79-80, 70-77 (2015-09-01)
The hydrolysis/fermentation of wheat straw and the adsorption/desorption/deactivation of cellulases were studied using Cellic(®) CTec2 (Cellic) and Celluclast mixed with Novozyme 188. The distribution of enzymes - cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase - of the two formulations
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service