CAT100
Catalase Assay Kit
sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells
Synonym(s):
Catalase Activity Detection Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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General description
The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.
Application
Sutitable for Colorimetric and UV Assays
Biochem/physiol Actions
Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.
Features and Benefits
- Useful for determining catalase activity - may be used in various tissues and cells
- Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity
Suitability
Suitable for studying catalase activity in various tissues and subcellular organelles.
Principle
This assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nm
Unit Definition
One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.
Preparation Note
Use ultrapure water in preparation of all solutions.
Kit Components Also Available Separately
Product No.
Description
SDS
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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M Zámocký et al.
Progress in biophysics and molecular biology, 72(1), 19-66 (1999-08-14)
This review gives an overview about the structural organisation of different evolutionary lines of all enzymes capable of efficient dismutation of hydrogen peroxide. Major potential applications in biotechnology and clinical medicine justify further investigations. According to structural and functional similarities
A J Kowaltowski et al.
FEBS letters, 473(2), 177-182 (2000-05-17)
The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae
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S Tada-Oikawa et al.
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Pulsed field gel electrophoresis showed that the initiation time of DNA breakage induced by the DNA alkylating agent duocarmycin A, which is not a redox-cycling agent, was almost the same in the human leukemia cell line HL-60 and its H2O2-resistant
Articles
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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