C5499
Cytochrome c Oxidase from bovine heart
5 mg protein/mL
Synonym(s):
EC 1.9.3.1, Ferrocytochrome-c:oxygen oxidoreductase, Complex IV
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About This Item
Recommended Products
biological source
bovine heart
Quality Level
form
buffered aqueous solution
specific activity
≥20 units/mg protein
mol wt
300-350 kDa by gel filtration
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
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concentration
5 mg protein/mL
greener alternative category
, Enabling
shipped in
dry ice
storage temp.
−20°C
General description
Research area: Cell cycle
Cytochrome c oxidase contains 13 subunits, encoded by mitochondrial genes and nuclear genome. It is comprised of two heme groups (Fea and Fea3) and two copper centers, CuA and CuB.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.
Cytochrome c oxidase contains 13 subunits, encoded by mitochondrial genes and nuclear genome. It is comprised of two heme groups (Fea and Fea3) and two copper centers, CuA and CuB.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.
Application
Cytochrome c Oxidase (CcO) from bovine heart has been used:
- to study the effect of oxidized glutathione (GSSG) on the number of total free cysteines
- in a histochemical assay to measure CcO activity in tissue sections
- to study the redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy
Biochem/physiol Actions
Cytochrome c oxidase is the principal terminal oxidase of high oxygen affinity in the aerobic metabolism of all animals, plants, yeasts and some bacteria. It is present in the mitochondria of the more highly developed cells and in the cytoplasmic membrane of bacteria. Cytochrome c oxidase catalyses the electron transfer from cytochrome c to O2. This electron-transfer process produces a proton gradient across the membrane, which in turn drives the production of ATP. This enzyme is unique in providing energy for the cell bycoupling the electron transport through the cytochrome chain with the process of oxidative phosphorylation.
Unit Definition
One unit of cytochrome c oxidase will oxidize 1.0 μmole of ferrocytochrome c per min. at 25 °C at pH 6.0.
Physical form
Supplied as a solution in 25 mM Tris-HCl buffer, pH 7.8, 5 mM EDTA, and 39 mM n-dodecyl β·-D-maltoside.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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