B1310

Anti-BACH1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-BRCA1-associated C-terminal helicase 1
• MDL number: MFCD06201924
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~150 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): 5-10 μg using RIPA extract (0.5 mg) of human HeLa cells; indirect immunofluorescence: 10-20 μg/mL using human MCF-7 cells; microarray: suitable; western blot: 0.5-1.0 μg/mL using whole extracts of MCF-7 human breast carcinoma cells
• UniProt accession no.: Q9BX63
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BRIP1(83990) ; mouse ... Brip1(237911) ; rat ... Brip1(360588)

Description

General description

BTB Domain And CNC Homolog 1 (BACH1) is expressed in numerous tissues. The protein includes a nuclear localization signal in its helical domain.

Immunogen

synthetic peptide corresponding to amino acid residues 1233-1249 of human BACH1 with N-terminal added cysteine, conjugated to KLH.

Application

Anti-BACH1 antibody produced in rabbit has been used in:

• immunoblotting
• immunohistochemistry
• western blotting

Polyclonal anti-BACH1 antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10 μg by using RIPA extract (0.5 mg) of human HeLa cells and for indirect immunofluorescence at 10-20 μg/mL using human MCF-7 cells. The product is also suitable for western blot analysis at a working concentration of 0.5-1.0 μg/mL by using whole extracts of MCF-7 human breast carcinoma cells. Further, anti-BACH1 antibody has shown suitability for microarray analysis.

Biochem/physiol Actions

BACH1 interacts directly with the C-terminal BCRT repeats in Breast cancer type1 protein (BRCA1), a tumor suppressor nuclear phosphoprotein that facilitates the repair of double-stranded DNA breaks. Bach1 also functions as transcription repressor in transfection assays using fibroblast cells and as a transcriptional activator and repressor, respectively, in cultured erythroid cells. Additionally, it is also involved in the coordination of transcription activation and repression by Maf family members such as MafK. Bach1 (BTB and CNC homology 1, basic leucine zipper transcription factor 1) inhibits oxidative stress-inducible genes and is a negative regulator of oxidative stress-induced cellular senescence.

BTB Domain And CNC Homolog 1 (BACH1) interaction between breast cancer type 1 (BRCA1) in vivo appears to be dependent on phosphorylation of Ser990 in the latter during the G2/M phase of the cell cycle. BACH1 mutations were found in patients with early-onset breast cancer. It displays a punctate nuclear staining pattern similar to that observed for BRCA1.

Target description

BACH1, BRCA1 interacting protein C-terminal helicase 1, encodes a protein that is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand br

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves