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  • Mitochondrial Respiration Is Decreased in Rat Kidney Following Fetal Exposure to a MaternalLow-ProteinDiet.

Mitochondrial Respiration Is Decreased in Rat Kidney Following Fetal Exposure to a MaternalLow-ProteinDiet.

Journal of nutrition and metabolism (2012-04-27)
Sarah Engeham, Kennedy Mdaki, Kirsty Jewell, Ruth Austin, Alexander N Lehner, Simon C Langley-Evans
ABSTRACT

Maternal protein restriction in rat pregnancy is associated with impaired renal development and age-related loss of renal function in the resulting offspring. Pregnant rats were fed either control or low-protein (LP) diets, and kidneys from their male offspring were collected at 4, 13, or 16 weeks of age. Mitochondrial state 3 and state 4 respiratory rates were decreased by a third in the LP exposed adults. The reduction in mitochondrial function was not explained by complex IV deficiency or altered expression of the complex I subunits that are typically associated with mitochondrial dysfunction. Similarly, there was no evidence that LP-exposure resulted in greater oxidative damage to the kidney, differential expression of ATP synthetase β-subunit, and ATP-ADP translocase 1. mRNA expression of uncoupling protein 2 was increased in adult rats exposed to LP in utero, but there was no evidence of differential expression at the protein level. Exposure to maternal undernutrition is associated with a decrease in mitochondrial respiration in kidneys of adult rats. In the absence of gross disturbances in respiratory chain protein expression, programming of coupling efficiency may explain the long-term impact of the maternal diet.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-ATP Synthase β antibody produced in mouse, 1 mg/mL, clone 4.3E8.D10, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-Uncoupling Protein-2 (UCP-2) (144-157) Rabbit pAb, liquid, Calbiochem®