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  • Generation of priming mesenchymal stem cells with enhanced potential to differentiate into specific cell lineages using extracellular matrix proteins.

Generation of priming mesenchymal stem cells with enhanced potential to differentiate into specific cell lineages using extracellular matrix proteins.

Biochemical and biophysical research communications (2013-06-12)
Na Rae Han, Jung Im Yun, Young Hyun Park, Ji Yeon Ahn, Choonghyo Kim, Jung Hoon Choi, Eunsong Lee, Jeong Mook Lim, Seung Tae Lee
ABSTRACT

Poor understanding of the differentiation of mesenchymal stem cells (MSCs) has resulted in a low differentiation yield, and has hindered their application in medicine. As a solution, priming MSCs sensitive to signaling, thus stimulating differentiation into a specific cell lineage, may improve the differentiation yield. To demonstrate this, priming MSCs were produced by using a gelatin matrix for the isolation of primary MSCs from bone-marrow-derived primary cells. Subsequently, cellular characteristics and sensitivity to specific differentiation signals were analyzed at passage five. Compared to non-priming MSCs, priming MSCs showed no significant differences in cellular characteristics, but demonstrated a significant increase in sensitivity to neurogenic differentiation signals. These results demonstrate that generation of priming MSCs by specific extracellular signaling increases the rate of differentiation into a cell-specific lineage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gelatin from bovine skin, gel strength ~225 g Bloom, Type B
Sigma-Aldrich
Gelatin from porcine skin, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
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Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
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Gelatin from bovine skin, Type B
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Gelatin, tested according to Ph. Eur.
Millipore
Gelatin from porcine skin, suitable for microbiology, high gel strength
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Gelatin from porcine skin, suitable for microbiology, low gel strength
Sigma-Aldrich
Gelatin from porcine skin, gel strength 80-120 g Bloom, Type A
Sigma-Aldrich
Gelatin from bovine skin, Type B, powder, BioReagent, suitable for cell culture
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Gelatin from porcine skin, gel strength ~175 g Bloom, Type A
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Gelatin from porcine skin, gel strength 300, Type A
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Prionex® Highly purified Type A, aqueous solution
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Gelatin from cold water fish skin, solid
Sigma-Aldrich
Gelatin solution, Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture
Millipore
Gelatin from porcine skin, medium gel strength, suitable for microbiology
Millipore
Gelatin from porcine skin, suitable for microbiology, ultrahigh gel strength