- Preparation and evaluation of natural scaffold materials for kidney regenerative applications.
Preparation and evaluation of natural scaffold materials for kidney regenerative applications.
Methods in molecular biology (Clifton, N.J.) (2013-03-16)
Monica A Serban, Toyin A Knight, Richard G Payne
PMID23494425
ABSTRACT
Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and integration. This biomaterial manufacturing technique is simple, cost-effective, highly reproducible and the in vivo evaluation procedure highly informative on the biocompatibility and regenerative potential of the tested construct.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Gelatin from porcine skin, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
Sigma-Aldrich
Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
Gelatin solution, Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture