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  • Preparation and evaluation of natural scaffold materials for kidney regenerative applications.

Preparation and evaluation of natural scaffold materials for kidney regenerative applications.

Methods in molecular biology (Clifton, N.J.) (2013-03-16)
Monica A Serban, Toyin A Knight, Richard G Payne
ABSTRACT

Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and integration. This biomaterial manufacturing technique is simple, cost-effective, highly reproducible and the in vivo evaluation procedure highly informative on the biocompatibility and regenerative potential of the tested construct.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gelatin, tested according to Ph. Eur.
Sigma-Aldrich
Gelatin from bovine skin, Type B
Sigma-Aldrich
Gelatin from porcine skin, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
Sigma-Aldrich
Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
Gelatin from bovine skin, gel strength ~225 g Bloom, Type B
Millipore
Gelatin from porcine skin, suitable for microbiology, high gel strength
Millipore
Gelatin from porcine skin, suitable for microbiology, low gel strength
Millipore
Gelatin from porcine skin, medium gel strength, suitable for microbiology
Sigma-Aldrich
Gelatin solution, Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture
Sigma-Aldrich
Gelatin from porcine skin, gel strength ~175 g Bloom, Type A
Sigma-Aldrich
Gelatin from bovine skin, Type B, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Gelatin from porcine skin, gel strength 300, Type A
Sigma-Aldrich
Gelatin from porcine skin, gel strength 80-120 g Bloom, Type A
Sigma-Aldrich
Gelatin from cold water fish skin, solid
Sigma-Aldrich
Prionex® Highly purified Type A, aqueous solution
Millipore
Gelatin from porcine skin, suitable for microbiology, ultrahigh gel strength