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  • Preparation and evaluation of natural scaffold materials for kidney regenerative applications.

Preparation and evaluation of natural scaffold materials for kidney regenerative applications.

Methods in molecular biology (Clifton, N.J.) (2013-03-16)
Monica A Serban, Toyin A Knight, Richard G Payne
ABSTRACT

Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and integration. This biomaterial manufacturing technique is simple, cost-effective, highly reproducible and the in vivo evaluation procedure highly informative on the biocompatibility and regenerative potential of the tested construct.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gelatin from bovine skin, gel strength ~225 g Bloom, Type B
Sigma-Aldrich
Gelatin from porcine skin, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
Sigma-Aldrich
Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
Gelatin from bovine skin, Type B
Sigma-Aldrich
Gelatin, tested according to Ph. Eur.
Millipore
Gelatin from porcine skin, suitable for microbiology, high gel strength
Millipore
Gelatin from porcine skin, suitable for microbiology, low gel strength
Sigma-Aldrich
Gelatin from cold water fish skin, solid
Sigma-Aldrich
Gelatin from porcine skin, gel strength ~175 g Bloom, Type A
Sigma-Aldrich
Gelatin from porcine skin, gel strength 300, Type A
Sigma-Aldrich
Gelatin from porcine skin, gel strength 80-120 g Bloom, Type A
Sigma-Aldrich
Gelatin from bovine skin, Type B, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Prionex® Highly purified Type A, aqueous solution
Sigma-Aldrich
Gelatin solution, Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture
Millipore
Gelatin from porcine skin, suitable for microbiology, ultrahigh gel strength
Millipore
Gelatin from porcine skin, medium gel strength, suitable for microbiology