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PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

Synonym(s):

Protease Assay Kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

shipped in

dry ice

storage temp.

−20°C

Application

Fluorescent Protease Detection Kit Video Protocol
Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

Suitability

Detection limit: approx. 50 ng trypsin

Quantity

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Analysis Note

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Kit Components Only

Product No.
Description
  • Incubation buffer 25 mL
  • FITC-casein solution 5 mL
  • Assay buffer 200 mL
  • Protease standard (trypsin) 20 μg
  • FITC control 50 mg
  • Trichloroacetic acid (TCA) solution 30 mL

Signal Word

Danger

Hazard Statements

H314,H317,H334,H335,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Virulence, 12(1), 584-600 (2021-02-05)
Using the USA300, methicillin-resistant Staphylococcus aureus strain LAC, we previously examined the impact of regulatory mutations implicated in biofilm formation on protease production and virulence in a murine sepsis model. Here we examined the impact of these mutations in the
M Lee et al.
Neuroscience, 229, 164-175 (2012-10-27)
Astrocytes become activated in degenerative neurological diseases. In order to gain a greater understanding of the inflammatory factors released upon activation, we stimulated adult human astrocytes with interferon-gamma and examined the resultant conditioned medium (CM) for toxicity against differentiated human
Marion F Cubitt et al.
Molecular plant-microbe interactions : MPMI, 26(3), 356-366 (2012-11-02)
Plant cell wall-degrading enzymes (PCWDE) are key virulence determinants in the pathogenesis of the potato pathogen Pectobacterium atrosepticum. In this study, we report the impact on virulence of a transposon insertion mutation in the metJ gene that codes for the
Fungal contamination of textile objects preserved in Slovene museums and religious institutions
Kavkler K, et al.
International Biodeterioration & Biodegradation, 97, 51-59 (2015)
Joseph S Rom et al.
Virulence, 8(8), 1776-1790 (2017-09-15)
Staphylococcus aureus causes acute and chronic forms of infection, the latter often associated with formation of a biofilm. It has previously been demonstrated that mutation of atl, codY, rot, sarA, and sigB limits biofilm formation in the USA300 strain LAC
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Better Protease Detection | High-Sensitivity Protease Detection Assay

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Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Protocols

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

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