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Key Documents

111A3

85062504, Application: Fusion partner in the production of heterohybridomas

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Bovine Heterohybridoma

ImmunoDon

Jersey calf lymph node

Myeloma

53B3 (mouse x bovine heteromyeloma)

Reactivity

Application: Fusion partner in the production of heterohybridomas

technique(s)

cell culture | mammalian: suitable

isotype

Not specified

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse 53B3 x Bovine Jersey Calf lymph node

Cell Line Description

Non Ig secreting

Immunogen

Sheep red blood cells

Application

This line has been used as a HAT sensitive fusion partner in the production of heterohybridomas

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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Articles

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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