5.37983

Pantothenate Kinase Inhibitor

• Synonym(s): Pantothenate Kinase Inhibitor, 3-(2,4-Dimethylpyrido-[2ʹ,3ʹ:3,4]pyrazolo[1,5-a]pyrimidin-3-yl)-N-(3-(methylthio)phenyl)propanamide, PanK Inhibitor
• Empirical Formula (Hill Notation): C₂₁H₂₁N₅O
• Molecular Weight: 359.42
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Assay: ≥97% (HPLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange
• solubility: DMSO: 10 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable pyridopyrazolopyrimidine based compound that acts as a potent and reversible inhibitor of pantothenate kinase (IC₅₀ = 70, 92 and 25 nM for PanK1β, PanK2 and PanK3, respectively; radiochemical assay, 2.5 mM [ATP]). Binds to ATP-PanK complex (apparent binding constant ~300 nM for ATP-PanK3) and exhibits mixed-type inhibition with respect to ATP and pantothenate. Shown to dose-dependently decrease [³H]pantothenate incorporation into coenzyme A (apparent IC₅₀ = 900 nM in C3A cells) with negligible off-target toxicity (IC₅₀ >56 µM in Hep-G2 cells).

A cell-permeable pyridopyrazolopyrimidine based compound that acts as a potent and reversible inhibitor of pantothenate kinase (IC₅₀ = 70, 92 and 25 nM for PanK1β, PanK2 and PanK3, respectively; radiochemical assay, 2.5 mM [ATP]). Binds to ATP-PanK complex (apparent binding constant ~300 nM for ATP-PanK3) and exhibits mixed-type inhibition with respect to ATP and pantothenate. Shown to dose-dependently decrease [³H]pantothenate incorporation into coenzyme A (apparent IC₅₀ = 900 nM in C3A cells) with negligible off-target toxicity (IC₅₀ >56 µM in Hep-G2 cells).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
pantothenate kinase

Reversible: yes

Target IC₅₀: 70, 92 and 25 nM for PanK1&beta

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Sharma, L.K., et al. 2015. J. Med. Chem.58, 1563.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable