A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8.
A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
Following initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.
Other Notes
Kojima, H., et al. 2001. J. Neurochem.76, 1404. Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876. Itoh, Y., et al. 2000. Anal. Biochem.287, 203. Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.
Legal Information
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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