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Merck
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Key Documents

1.05174

Sigma-Aldrich

Hematoxylin solution modified acc. to Gill III

for microscopy

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About This Item

UNSPSC Code:
41116124
NACRES:
NA.41

Quality Level

form

liquid

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

pH

2.5 (20 °C in H2O)

density

1.07 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

Hematoxylin solution modified according to Gill III - for microscopy, is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin. It is a ready-to-use staining solution that when used together with other in vitro diagnostic products from our portfolio makes target structures evaluable for diagnostic purposes in histological and clinico-cytological specimen materials, for example histological sections of e. g. the kidney, muscle tissue, heart, or lung. In hematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready-to-use staining solutions, such as Mayer′s hemalum solution or Hematoxylin solution modified according to Gill III. These solutions do not need to be filtrated prior use and the dye is oxidized in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e. for proteins, collagen, keratin, intercellular substances. can be performed with alcoholic or aqueous Eosin Y solution 0.5 %.
A 500 ml bottle is sufficient for 1500 - 2500 stainings. It is registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Nuclei: dark blue to dark violet
Cytoplasm: pink to red
intercellular substances: pink to red
Erythrocytes: yellow to orange

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - STOT RE 2 Oral

Target Organs

Kidney

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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