Inactivation of Burkholderia pseudomallei on environmental surfaces using spray-applied, common liquid disinfectants.

Inactivation of Burkholderia pseudomallei on environmental surfaces using spray-applied, common liquid disinfectants.

Letters in applied microbiology (2015-09-04)

M W Calfee, M Wendling

ABSTRACT

Five commercially available liquid antimicrobials were evaluated for their ability to decontaminate common environmental surface materials, contaminated with Burkholderia pseudomallei, using a spray-based disinfectant delivery procedure. Tests were conducted at both an ambient temperature (c. 20°C) and a lower temperature (c. 12°C) condition. Nonporous materials (glass and aluminium) were more easily decontaminated than porous materials (wood, concrete and carpet). Citric acid (1%) demonstrated poor efficacy in all test conditions. Bleach (pH-adjusted), ethanol (70%), quaternary ammonium and PineSol®, demonstrated high (>6 log10 reduction) efficacies on glass and aluminium at both temperatures, but achieved varying results for wood, carpet and concrete. Temperature had minimal effect on decontamination efficacy during these tests. Much of the antimicrobial efficacy data for pathogenic micro-organisms are generated with testing that utilizes hard nonporous surface materials. These data are not directly translatable for decontaminant selection following an incident whereby complex and porous environmental surfaces are contaminated. This study presents efficacy data for spray-applied antimicrobial liquids, when used to decontaminate common environmental surfaces contaminated with Burkholderia pseudomallei. These data can help responders develop effective remediation strategies following an environmental contamination incident involving B. pseudomallei.

MATERIALS

Product Number

Brand

Product Description

733369

Sigma-Aldrich

Aluminum, foil, thickness 8 μm, 99% trace metals basis

S4701

Sigma-Aldrich

Suplatast tosylate, ≥98% (HPLC)

11009

Sigma-Aldrich

Aluminum, powder, ≥91% (complexometric)

433705

Sigma-Aldrich

Aluminum, evaporation slug, diam. × L 6.3 mm × 6.3 mm, 99.999% trace metals basis

326887

Sigma-Aldrich

Aluminum, wire, diam. 0.58 mm, ≥99.99% trace metals basis

326941

Sigma-Aldrich

Aluminum, pellets, 3-8 mesh, ≥99.999% trace metals basis

518573

Sigma-Aldrich

Aluminum, granular, <1 mm, 99.7% trace metals basis

266523

Sigma-Aldrich

Aluminum, ReagentPlus^®, beads, 5-15 mm, 99.9% trace metals basis

GF03467202

Aluminum, honeycomb, 300x300mm, thickness 52.3mm, cell size 6.3mm, cell wall 0.064mm, core density 0.083g/cm³, phenolic adhesive (resin), facing skin glass fiber 0.75mm, 99.9%

GF33400224

Aluminum, honeycomb, 600x600mm, thickness 52.3mm, cell size 6.3mm, cell wall 0.064mm, core density 0.083g/cm³, phenolic adhesive (resin), facing skin glass fiber 0.75mm, 99.9%

GF10029596

Aluminum, foam, 150x150mm, thickness 6.35mm, bulk density 0.2g/cm³, porosity 0.93, 8 pores/cm, aluminum 6101 grade, 98.5%

GF96312326

Aluminum, honeycomb, 600x600mm, thickness 26.5mm, cell size 6.3mm, cell wall 0.064mm, core density 0.083g/cm³, phenolic adhesive (resin), facing skin carbon fiber / epoxy, 99.9%

GF36470178

Aluminum, honeycomb, 300x300mm, thickness 5mm, cell size 13mm, cell wall 0.1mm, core density 0.062g/cm³, phenolic adhesive (resin), 99.5%

GF24569602

Aluminum, foam, 150x150mm, thickness 30mm, bulk density 0.2g/cm³, porosity 0.93, aluminum 6101 grade, 98.5%

GF26773230

Aluminum, honeycomb, 300x300mm, thickness 13.9mm, cell size 6.3mm, cell wall 0.064mm, core density 0.083g/cm³, phenolic adhesive (resin), facing skin aluminum 0.55mm, 99.9%

GF86118969

Aluminum, honeycomb, 300x300mm, thickness 5mm, cell size 19mm, cell wall 0.064mm, core density 0.029g/cm³, phenolic adhesive (resin), 99.5%

GF72634103

Aluminum, rod, 50mm, diameter 28mm, as drawn, 99.999%

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Aluminum, rod, 100mm, diameter 6.0mm, as drawn, 99.999%

GF98025239

Aluminum, tube, 100mm, outside diameter 0.55mm, inside diameter 0.4mm, wall thickness 0.075mm, as drawn, 99.5%

GF04709695

Aluminum, rod, 44mm, diameter 77.5mm, as drawn, 99.999%

GF02208923

Aluminum, tube, 200mm, outside diameter 0.86mm, inside diameter 0.76mm, wall thickness 0.05mm, as drawn, 99.5%

GF87559420

Aluminum, tube, 0.5m coil, as drawn, outside diameter 0.30mm, inside diameter 0.2mm, wall thickness 0.05mm, 99.5%

GF49694743

Aluminum, tube, 100mm, outside diameter 0.6mm, inside diameter 0.3mm, wall thickness 0.15mm, as drawn, 99.5%

GF94706354

Aluminum, mesh, 150x150mm, nominal aperture 0.38mm, wire diameter 0.25mm, 40x40 wires/inch, open area 37%, plain weave mesh

GF93309037

Aluminum, tube, 200mm, outside diameter 1.42mm, inside diameter 1.166mm, wall thickness 0.127mm, as drawn, 99.5%

GF06197014

Aluminum, rod, 200mm, diameter 10.0mm, as drawn, 99.999%

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Aluminum, tube, 500mm, outside diameter 0.55mm, inside diameter 0.4mm, wall thickness 0.075mm, as drawn, 99.5%

GF16562246

Aluminum, tube, 500mm, outside diameter 3.0mm, inside diameter 1.62mm, wall thickness 0.69mm, hard, 99.5%

GF98718935

Aluminum, sphere, 100pcs, diameter 1.0mm, aluminum alloy 2017 precision sphere, grade 200, 95.2%

GF51598761

Aluminum, rod, 200mm, diameter 12.7mm, 99%